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KMID : 0606920110190020149
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Biomolecules & Therapeutics
2011 Volume.19 No. 2 p.149 ~ p.154
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Application of MALDI Tissue Imaging of Drugs and Metabolites: A New Frontier for Molecular Histology
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Shanta Selina Rahman
Kim Young-Jun
Kim Young-Hwan
Kim Kwang-Pyo
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Abstract
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Matrix assisted laser desorption ionization (MALDI) mass spectrometry is commonly used to analyze biological molecules such as proteins, peptides and lipids from cells or tissue. Recently MALDI Imaging mass spectrometry (IMS) has been widely applied for the identifi cation of different drugs and their metabolites in tissue. This special feature has made MALDI-MS a common choice for investigation of the molecular histology of pathological samples as well as an important alternative to other conventional imaging methods. The basic advantages of MALDI-IMS are its simple technique, rapid acquisition, increased sensitivity and most prominently, its capacity for direct tissue analysis without prior sample preparation. Moreover, with ms/ms analysis, it is possible to acquire structural information of known or unknown analytes directly from tissue sections. In recent years, MALDI-IMS has made enormous advances in the pathological fi eld. Indeed, it is now possible to identify various changes in biological components due to disease states directly on tissue as well as to analyze the effect of treated drugs. In this review, we focus on the advantages of MALDI tissue imaging over traditional methods and highlight some motivating fi ndings that are signifi cant in pathological studies.
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KEYWORD
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MALDI imaging, Metabolites, Lipids, Drugs
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